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文献和实验SSR GEL and Silver Staining Protocol
cylinder and add 10 ml 10x TBE, bringing volume to 100 ml with d.i. water. 4. We have recently started using Burst-Pack from Owl Scientific, which is an acrylamide premix including the buffer and catalysts, for our fluorescent gel work and are very pleased
, 2.2M dNTP’s 100mM EDTA 0.5M EtBr 10mg/ml Gelatin 2% Glucose 1; 1.5; 2M Guanidine HCl 1-8M HEPES 1M Imidazol 2M Paraformaldehyde 37% PEG 40% PMSF 100mM Retinoic acid 10mM Sucrose 1; 2; 2.5M Tris Cl 1M
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
speed. 4. Aspirate supernatant, resuspend pellet in 20ml ice cold 1mM HEPES pH7 (sterile filtered), respin. 5. Repeat 4. two more times! 6. After aspirating, resuspend pellet in 2ml ice cold 10% glycerol (sterile filtered). 7, ASAP
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