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文献和实验DNeasy 96植物技术:使用搅拌磨MM 300从新鲜植物叶片中分离DNA
DNeasy® 96 Plant Protocol for Isolation of DNA from Fresh Plant Leaves Using the Mixer Mill MM 300 一、Important notes before starting (使用前的重要注释) 1、Take time to familiarize yourself with the Mixer Mill MM 300 and the Centrifuge 4-15C/ Centrifuge 4K15C
96 Well Mating Protocol platewise
on the XGAL plate 2. Streak recipient plasmid (pMSCV) on LB Spec50 CB100 0.2% glucose OR YEGlu spec50 cb100) Grow at 30oC overnight Protocol 1. Inoculate 5 ml single colony culture of recipient, and a 1:100 donor culture in 500 ul/well 96 well culture box
96 Well Mating Protocol platewise
+Spec50+CB100+0.2% glucose OR YEGlu+spec50+cb100) Grow at 30oC overnight Protocol1. Inoculate 5 ml single colony culture of recipient, and a 1:100 donor culture in 500 ul/well 96 well culture box. Grow recipient at 30oC and donor at 37℃, shaking
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