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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
tip) alternatively: Finger controlled pipette (Manostat tubing, yellow tip, scalpel blade); 9'' Pasteur pipettes; 70% Ethanol; Alcohol burner or Bunsen burner; Sterile plastic Petri dishes (100 x 15 mm); Sterile
media. 6 3. Insert the tip of a sterile pipette into the stock bottle and remove 10 ml of media. Transfer the media to the tissue culture flask. 4. Open the top of the suspension culture and use a sterile 1.0 ml transfer pipette to remove a 1.0 ml
Purification of acidic phosphatase from mustard seedlings
1 - Day 1 1. Remove the 5 - 10 cm long seedlings from the vermiculite, wash and pat dry on paper towel. Weigh and record the weight. (use 25-50 g). 2. Grind in a mortar with 75 ml of ice-cold water
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