SEROLOGICAL PIPETTE 10ML, 25/B

原创：western protocol

. 4.  When finished, immerse membrane in Blocking buffer and block overnight. H. Antibodies and detection 1.  Incubate with primary antibody diluted in Blocking buffer for 2 hours at room temp. 2.  Wash 3 x 10 min with PBST. 1 x10min secondary washing

Cell Cultures

with PBS. 7. Mince the embryo finely with scissors and transfer the minced tissue to a flask containing PBS. Allow the tissue pieces to settle. 8. Remove the PBS with a sterile pipette and add 25 ml of trypsin, a proteolytic enzyme. Stir the solution

Establishment of a Primary Culture

containing PBS. Allow the tissue pieces to settle.   Remove the PBS with a sterile pipette and add 25 ml of trypsin, a proteolytic enzyme. Stir the solution gently at 37° C for 15-20 minutes.   Allow the larger