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文献和实验RNAase Dissolve in 50 mM KAC pH 5.5 Heat to 100 o C for 15 minutes and cool slowly to room temperature Store at -20. (common freezer for stock solutions) 上一篇
Purification of Plasmid from 50 ml-culture
1. Shake E. coli harboring plasmid at 37 C overnight in 50 ml of TB containing appropriate antibiotics. (when using ampicillin, addition of the antibiotics to 100-200 ug/ml rather than usual 50 ug/ml may improve the yield of plasmids.)
SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
实验试剂 1. Isopropanol (100%) 2. 70% ethanol 3. Using different volume of Solution according blood volume as following: 实验设备 1. Centrifuge capable of 2,000 x g 2. Nuclease-free
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