6 WELL PLATE ADVANCED TC STERI

小RNA的Northern BLOT

!!)  UV shadow gel with TC plate and take a picture to ensure samples are evenly loaded (although will eventually want to reprobe blot with a loading control).  Put a piece of Whatman 3M paper (pre-wet with 1x TBE) on gel, put glass plate

Use of the Sleeping Beauty Transposon System for Stable Gene Expression in Mouse Embryonic Stem Cells

, and flasks (sterile tissue culture, gelatin-coated; 48-well, 24-well, 12-well, six-well, 10-cm, T25) Prepare gelatin-coated plasticware just before use by coating the bottom of each dish with 0.1% (w/v) gelatin solution (Sigma). Incubate the dishes

General Laboratory Procedures, Equipment Use, and Safety Considerations

should always be done on fresh cultures, either from a freshly streaked plate or from a glycerol stock. To grow a small scale E. coli culture, prepare 3-5 ml of LB (or appropriate broth - include antibiotic if the culture contains a plasmid) in two sterile 50 ml tubes. (Note