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文献和实验Worm PCR Pick one worm and place it in a 2.5 l drop of lysis buffer in the cap of a PCR tube. Close and centrifuge briefly to move to the bottom of the tube. Freeze the tubes at -70°C for 15 min. The idea is to do a freeze
isolated from the other components. PCR products should be analyzed in an area separate from the reaction assembly area. 1) Add the following components to a sterile 0.5-mL microcentrifuge tube on ice: 2) Cap the tubes and centrifuge briefly
(BD Biosciences / Clontech). SYBR Green PCR master mix, 200 reactions (Applied Biosystems). Optical tube and cap strips (Applied Biosystems). SuperScript
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