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文献和实验Gene Knockout with Conventional Mutagens
on your bench until the worms have settled to the bottom. with a Pasteur pipet attached to vacuum, suck off liquid so that you have about 1.5 ml left in each tube. (This is the step I worry about -- I'm afraid if the worms sit
for 7 minute. Inner PCR : 4.1 10 µl of outer PCR product is diluted into 990 µl H2O in eppendorf tube. The dilution is mixed by vortex followed by a short spin in a microfuge to make certain that no diluent was attached to the inner ring of the lid
DNAExtractionProtocolsUsingSilica
tubes Aluminum Foil 10M HCl I. Preparation of solutions A. Silica - note: 2 day procedure 1. Add 6 g SiO2 to a 50 mL Falcon tube, and fill to the 50 mL mark with deionized water (dH2O). 2. Vortex and allow to settle 24 h at room
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