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文献和实验Partial proteolysis with Staph. aureus V8 protease
Materials: Overlay/Rehydration buffer: 125 mM Tris-HCl, pH 6.8 1mM EDTA 0.1% SDS 1mM 2-b-mercaptoethanol 30% glycerol some Bromophenol blue For 1 ml, you will need 0.35 ml of 2X buffer 0.30 ml H20
In Situ Hybridization Using Digoxigenin Labeled Probes
morphology. After the pretreatment the slides should be hybridized within 24 hours. 1) Place the slides in 2X SSC at 65°C for 30 minutes 2) Wash for 2 minutes in 2X SSC at room temperature. 3) Acetylate the slides as follows: Prepare 500 ml of 0.1 M
A safe method of extracting DNA from Coccidioides immitis
in the saprophytic phase at 37 C in 100 ml of 2X GYE medium (20 g glucose and 10 g yeast extract in 1 liter medium, autoclaved for 15 min.) in 500 ml plastic Nalgene flasks with plastic foam stoppers (Identi-plug, Fisher). When there has been sufficient growth
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