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- BIO-RAD
- TCS0801
- 美国
- 2025年07月23日
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- 供应商：
BIO-RAD
- 规格：
TCS0801
Use domed cap strips for sealing of all Bio-Rad plates and 0.2 ml PCR tubes during thermal cycling.
- Very tight sealing
- Use for cold storage
- Free of DNase, RNase, and human DNA
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文献和实验Worm PCR Pick one worm and place it in a 2.5 l drop of lysis buffer in the cap of a PCR tube. Close and centrifuge briefly to move to the bottom of the tube. Freeze the tubes at -70°C for 15 min. The idea is to do a freeze
(BD Biosciences / Clontech). SYBR Green PCR master mix, 200 reactions (Applied Biosystems). Optical tube and cap strips (Applied Biosystems). SuperScript
PCR Amplification from Microbial Colonies
min and voltex. Spin (14 K, 5 min). Withdraw 2 µl of the sample (supernatent), add to 16 µl H2O in the 0.2 ml tube, and add a mixture of 2.5 µl 10X PCR buffer, 2 µl 2.5 µM primer , 2 µl 2.5 mM dNTP (2.5 mM each of dATP, dCTP, dGTP, and dTTP
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