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文献和实验Staining protocols for placental explant cultures
-cytokeratin 7 (OV-TL 12/30; Dako; use at 1/50). This identifies the outgrowing cells unequivocally as trophoblast. Anticytokeratin 8/18 can be used for marking cells (e.g CAM5.2, Beckton-Dickenson) but beware that some placental fibroblasts express this marker
Staining protocols for placental explant cultures
oxide 2 x 15 min, in a fume cupboard, wearing protective gloves. Infiltrate in equal parts epoxy resin and propylene oxide for 1 h (Epon, Araldite, Taab Embedding Resin or other epoxy resin mixtures can be used). This must be done in a fume
.) Dehydrate in 90% ethanol - 10 mins. x 2. Dehydrate in 100% ethanol - 20 mins. x 2. Propylene oxide (1.2 epoxy propane) - 10 mins. x 2. Propylene oxide/epoxy resin mixture (50/50) - 1 hour. Epoxy resin - overnight
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