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文献和实验Kamps's Western Blotting Protocol
activity).We use 12-14 microCi (approx.10 microliters)125I-protein A in a volume of 30-35 ml blocking buffer.We reuse this solution 3 times without noticing a reduction in signal (see #6 in Comments). 9)Wash again as in step 7. Damp dry on Whatman 3MM
James Hardwick's angiotensin assay protocol
that you use for precipitation or you will risk losses. 1.Immunoprecipitate the protein from 3 x106 cells using 45 microliters S.aureus bugs.Resuspend the immunoprecipitates in 12 microliters kinase buffer (40 mM PIPES,pH 7.0,10 mM MnCl2)at 4℃. 2.The kinase
C. elegans whole mount immunohistochemistry with Bouin's Fixative
fixed worms (e.g. the Finney protocol). A Bouin's fixative of 75 ml saturated picric acid [real nasty stuff- explosive in crystal form!], 25 ml of formalin, and 5 ml of glacial acetic acid is made (many fly labs use this fixation solution
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