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Epicentre在不断研发新的试剂用于基因组学、蛋白组学和RNA研究等方面,使您能够获得更多更好的实验解决方案。我们希望您能联系我们并告知您关于新产品的研究需求和想法。下面是最新的产品清单:
逆转录酶 EpiScript™ Reverse Transcriptase
Reverse Transcription and cDNA Synthesis | cDNA Synthesis 逆转录和cDNA合成
EpiScript™ Reverse Transcriptase (EpiScript RT), an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. EpiScript RT demonstrates significantly more activity than other MMLV Reverse transcriptase enzymes. It is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR (qRT-PCR) analysis and other applications.
rRNA移除试剂盒 Globin-Zero™ Gold Kit
RNA Sequencing | rRNA Removal RNA测序、rRNA移除
The Globin-Zero™ Gold Kit enriches for non-globin coding and non-coding RNAs in blood-derived RNA samples. A Globin-Zero kit reaction efficiently removes the highly abundant adult globin mRNAs and the cytoplasmic and mitochondrial rRNAs from RNA isolated from human, mouse or rat blood for RNA-Seq library preparation and other applications.
ScriptSeq™ Complete Gold Kit (Blood)
RNA Sequencing | RNA Library Prep
The ScriptSeq™ Complete Gold Kits (Blood) generate RNA-Seq libraries from whole blood RNA in less than 1 day. The Kits include Epicentre's powerful Globin-Zero™ technology that removes virtually all the globin mRNA and ribosomal RNA (rRNA) from the sample to enable preparation of high complexity libraries for deep sequencing.
ScriptSeq™ Complete Kit (Plant)
RNA Sequencing | RNA Library Prep
Generate directional RNA-Seq libraries, virtually free of rRNA, from as little as 100 ng of intact or partially degraded plant leaf and plant seed/root RNA, in less than one day for Illumina® sequencing.
ARTseq™ Ribosome Profiling Kits
RNA Sequencing | Ribosome Profiling
The ARTseq™ Kit produces RNA-Seq libraries from ribosome-protected mRNA.
Ribo-Zero™ Magnetic Gold Kit (Epidemiology)
RNA Sequencing | rRNA Removal
The Ribo-Zero™ Magnetic Gold Kit (Epidemiology) combines Epicentre's Ribo-Zero™ Gold (Human/Mouse/Rat) and Ribo-Zero™ (Bacteria) rRNA removal reagents into a single solution that effectively removes rRNA, including human mitochondrial RNA, from samples composed of both human/mouse/rat and bacterial RNA.
ScriptSeq™ Complete Gold Kit (Epidemiology)-Low Input
ScriptSeq™ Complete Gold Kit (Epidemiology)
RNA Sequencing | RNA Library Prep
The ScriptSeq™ Complete Kit (Epidemiology) combines Epicentre's Ribo-Zero™ Gold (Epidemiology) rRNA removal reagents and ScriptSeq v2 RNA-Seq Library Preparation Kit for seamless end-to-end RNA-Seq library prep from samples composed of both human/mouse/rat and Gram-negative and Gram-positive bacterial RNA.
ScriptSeq™ Complete Kit (Bacteria)-Low Input
ScriptSeq™ Complete Kit (Bacteria)
RNA Sequencing | RNA Library Prep
The ScriptSeq™ Complete Kit (Bacteria) combines Epicentre's powerful Ribo-Zero™ technology and rapid ScriptSeq v2 RNA-Seq library prep procedure for optimized comprehensive preparation of RNA-Seq libraries in 1 day.
ScriptSeq™ Index PCR Primers
RNA Sequencing | RNA Library Prep
The ScriptSeq™ Index PCR Primers are used to add an Illumina® Index (barcode) to an RNA-Seq library prepared using Epicentre's ScriptSeq™ v2 RNA-Seq Library Preparation Kit.
ScriptSeq™ Complete Kits (Human/Mouse/Rat)
RNA Sequencing | RNA Library Prep
The ScriptSeq™ Complete kits combine Epicentre's powerful Ribo-Zero™ technology and rapid ScriptSeq v2 procedure for seamless, end-to-end preparation of RNA-Seq libraries in 1 day.
Ribo-Zero™ Magnetic Kit (Gram-Negative Bacteria)
RNA Sequencing | rRNA Removal
The new Ribo-Zero™ rRNA Removal Kit (Gram-Negative Bacteria) removes virtually all the ribosomal RNA (rRNA), including the difficult-to-remove 5S rRNA, from intact and partially degraded Gram-negative bacterial total RNA preparations.
Ribo-Zero™ Magnetic Kit (Gram-Positive Bacteria)
RNA Sequencing | rRNA Removal
The new Ribo-Zero™ rRNA Removal Kit (Gram-Positive Bacteria) removes virtually all the ribosomal RNA (rRNA) from intact and partially degraded Gram-positive bacterial total RNA preparations.
TargetAmp™-Pico Labeling Kit for Illumina® Expression BeadChip®
Array-Based Analysis | RNA Amplification for Microarrays
The TargetAmp™-Pico Labeling Kit for Illumina® Expression BeadChip® is the only kit that can produce micrograms amounts of biotin-aRNA (also called cRNA) from as little as 50 pg of total RNA for use on Illumina Expression BeadChip arrays. The kit uses a significantly improved Eberwine linear RNA amplification method, but incorporates a number of improvements that significantly increase the yields of biotin-aRNA obtained, while reducing the level of nonspecific RNA amplification products.
详见 www.epicentre.cn 北京中北林格科技发展有限公司 www.bjzblg.com 010-88891086
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文献和实验Cell Stem Cell:邓宏魁课题组建立更加快速高效的人体细胞化学重编程体系
;而化学重编程是利用外源的化学小分子模拟外界信号刺激,驱动细胞命运以分阶段的方式发生转变。因此,该方法可控性强,有望实现精准调控细胞命运、逆转细胞身份和功能状态,使逆向发育成为可能。 2013 年,北京大学邓宏魁研究组在 Science 杂志发表了一项原创性的成果,即不依赖卵母细胞和转录因子等细胞内源物质,仅使用外源性化学小分子就可以逆转细胞命运,将小鼠体细胞重编程为多潜能干细胞(chemically induced pluripotent stem cells,CiPS 细胞),开辟了一条全新
6 篇 Science 齐发!首次破译完整的人类基因组,百人科学家团队揭开背后的神秘面纱
步,人类参考基因组不断完善,已经更新了许多版本。其中,人类参考基因组 GRCh38 自 2013 年首次发布以来,作为极具价值的研究工具被广泛使用,但确定复杂基因组区域的确切序列一直是一项具有挑战性的工作。本次更新的参考基因组 T2T-CHM13,在 GRCh38 的基础上增加了近 2 亿个碱基对,填补了剩余的 8% 的空白,为我们提供了第一个完整的 DNA 视图。 过去,我们已经从拥有的大约 90% 的人类基因组中获得了对人类生物学和疾病的深刻理解,而新破译的 DNA 序列将彻底改变我们对人类
相关专题 RNA提取 从RNA合成到RNA干扰实验技术 近日,加州大学伯克利分校的科学家新研发出一种RNA提取实验方案,这种方法适用于所有陆生植物(包括30万物种)。这一实验方案将大大推动非模式植物物种以RNA为基础的研究。相关文章发表于2013年12月9日的《Applications in Plant science s》杂志上。 加州大学推出一种适用于所有陆生植物的RNA提取 新方法 RNA在遗传
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