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- 欣润生物（NEWGAINBIO)
- 江苏无锡
- IR3001
- 2025年10月29日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Immortalized rat myocardial fibroblasts
- 库存：
100万
- 供应商：
欣润生物
- 肿瘤类型：
否
- 细胞类型：
永生化大鼠心肌成纤维细胞
- ATCC Number：
无
- 品系：
SD大鼠
- 组织来源：
心肌
- 相关疾病：
无
- 物种来源：
大鼠
- 免疫类型：
不详
- 细胞形态：
成纤维细胞样
- 是否是肿瘤细胞：
否
- 器官来源：
心肌
- 运输方式：
常温
- 年限：
新生鼠
- 生长状态：
贴壁生长
- 规格：
T25方瓶
产品介绍
细胞名称：永生化大鼠心肌成纤维细胞、大鼠心肌成纤维细胞系
背景描述：大鼠心肌成纤维细胞系来源于大鼠心脏组织，心脏是脊椎动物身体中最重要的一个器官，主要功能是提供压力，把血液运行至身体各个部分。心脏的作用是推动血液流动，向器官、组织提供充足的血流量，以供应氧和各种营养物质，并带走代谢的终产物（如二氧化碳、无机盐、尿素和尿酸等），使细胞维持正常的代谢和功能。心脏中，大鼠心肌成纤维细胞系约占正常心肌组织细胞总数的60%-70%，是心脏中非心肌细胞的主要组成部分，广泛存在于心脏组织中，包围心肌细胞，连接心肌细胞间质，与缺血性心脏病、炎症、肥大、梗死等病理状态密切相关。。
产品货号：IR3001
细胞类型:永生化细胞系
传代能力:可传代30代左右
细胞形态:成纤维细胞样
培养基：大鼠心肌成纤维细胞系完全培养基
支原体分析：阴性
培养条件：37℃，5%CO2
发货方式：T25方瓶
货期：1周左右时间
Vimentin和Fibronectin免疫荧光检测呈阳性
Immortalization of rat embryo fibroblasts by the cellular p53 oncogene.
Intact and rearranged genes from Friend virus-induced erythroleukemic cell lines which code for proteins of 53- () and 44-kDa (), respectively, were cloned into pUC18 and tested for their ability to immortalize embryo fibroblasts. The functional gene from normal Balb/c liver was also tested for immortalizing activity. Immortal cells were obtained with the three genes although the efficiency of immortalization by was lower than that by . Expression of and in the established cell lines was confirmed by metabolic labeling and Western Blotting. Our results demonstrate that elevated expression of the gene, driven by its natural promoter and in the absence of strong heterologous promoters and/or enhancers, can efficiently immortalize early-passage embryo fibroblasts. -immortalized cells but not -immortalized cells could be morphologically transformed by secondary transfection with activated . Thus 5'-coding sequences of the gene appear necessary for complementation but not for immortalization.
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- 作者
- 内容
- 询问日期
文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
技术资料