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- 欣润生物（NEWGAINBIO)
- 江苏无锡
- IM2018
- 2025年12月09日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Immortalized mouse bladder fibroblasts
- 库存：
100万
- 供应商：
欣润生物
- 肿瘤类型：
否
- 细胞类型：
永生化
- ATCC Number：
无
- 品系：
ICR
- 组织来源：
膀胱
- 相关疾病：
无
- 物种来源：
小鼠
- 免疫类型：
不详
- 细胞形态：
成纤维细胞样
- 是否是肿瘤细胞：
否
- 器官来源：
膀胱
- 运输方式：
常温
- 年限：
/
- 生长状态：
贴壁生长
- 规格：
T25方瓶
产品简介
细胞名称：永生化小鼠膀胱成纤维细胞、小鼠膀胱成纤维细胞系
背景描述：小鼠膀胱壁主要由三层组织组成的，由内外为粘膜层、肌层和外膜。其中肌层由平滑肌构成。体外培养永生化小鼠膀胱成纤维细胞、小鼠膀胱成纤维细胞系不仅为组织工程膀胱，尿道提供种植细胞的必要手段，也是研究平滑肌瘤的基础与前提。。
产品货号：IM2018
细胞类型:永生化细胞
传代能力:可传代30代左右
细胞形态:梭形
培养基：永生化小鼠膀胱成纤维细胞、小鼠膀胱成纤维细胞系完全培养基
支原体：阴性
培养条件：37℃，5%CO2
发货方式：T25方瓶
货期：1周左右时间
FSP1免疫荧光检测呈阳性
The isolation of an immortalized and tumorigenic cell line from p21WAF1 null mouse bladders
Given a role for the deregulation of p21WAF1 in the progression of bladder tumors, we examined the growth of cultured urothelial cells from wild-type and p21WAF1 null bladders. Bladders were excised, minced from euthanized p21WAF1 and wild-type mice, treated overnight with dispase, and then placed into flasks coated with collagen type I in Dulbecco modified Eagle medium with 10% fetal calf serum. After an overnight incubation, the media was replaced with a serum-free media and a portion of explants were treated with 12-O-tetrade-canoylphorbol-13-acetate (TPA) on day 7 and continued for either 4 or 9 wk. The urothelial origin of any surviving epithelial cells was determined by reverse transcription-polymerase chain reaction (RT-PCR) using uroplakin II-specific primers, and the expression of the cell cycle-related proteins, p161NK4 and p19ARF, was examined by semiquantitative RT-PCR and Western blotting. Isolated wild-type and serially passaged p21WAF1 null epithelial-like cells were then injected subcutaneously into nude mice. We found that phorbol
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文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
为同一类型的细胞，如上皮细胞或成纤维细胞，也仍具有异质性，在分析细胞生物学特性时比较困难。其次，由于供体的个体差异及其他一些原因，细胞群生长效果有时也不一致。原代培养也是建立各种细胞系（株）必经的阶段。假如原代培养能够维持几小时甚至更长，即可进行进一步筛选。有的细胞具有继续增殖能力，有的细胞类型只是存活而不增殖，而另外一些细胞只是在特殊条件下应用而不存活，因而细胞类型的分布将会改变。在单层培养的情况下，瓶底全部铺满细胞，达到会合以后，对密度有依赖性的细胞则逐渐减少生长，而失去密度依赖敏感性的细胞
相关专题 肿瘤细胞培养 与常规的细胞培养技术相同，最特殊的在于成纤维细胞的排除，成纤维细胞与肿瘤细胞混合生长，无法纯化肿瘤组织。那么肿瘤细胞的生物学特性是什么呢？有哪些方法可以成功消除成纤维细胞呢？ 肿瘤细胞在组织培养中占有核心的位置，首先癌细胞是比较容易培养的细胞。当前建立的细胞系中癌细胞系是最多的。另外肿瘤对人类是威胁最大的疾病。肿瘤细胞培养 是研究癌变机理、抗癌药检测、癌分子生物学极其重要的手段。肿瘤
干细胞培养时易于生长增殖；把干细胞分离出来的培养方法称干细胞培养。（六）细胞遗传大多数肿瘤细胞有遗传学改变，如失去二倍体核型、呈异倍体或多倍体等。肿瘤细胞群常由多个细胞群组成，有干细胞系和数个亚系，并不断进行着适应性演变。（七）其它肿瘤细胞在体外不易生长的原因可能由于：①依赖性：肿瘤细胞虽有较强克隆生长力，但仍有一定的群体性或与其它细胞相依存关系。一是肿瘤细胞与肿瘤细胞的相互依存，二是肿瘤细胞与基质成纤维细胞的依赖。体外分散培养和排除成纤维细胞后也会同时消除或减弱这些依存关系，可能影响癌细胞增殖生长的活性
