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- 翌圣生物（Yeasen）
- 93358ES60
- 上海
- 2026年01月02日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 英文名：
Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein,hFc Tag
- 保质期：
一年
- 供应商：
翌圣生物科技（上海）股份有限公司
- 保存条件：
-20度
- 规格：
100μg
| 表达区间及表达系统（Source） | Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Asp23-Asp126 (CD3E)&Gln23-Ser116(CD3G).[uniprot | P07766(CD3E)&P09693(CD3G)] |
| 分子量大小（Molecular Weight） | The protein has a predicted MW of 37.8 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result. |
| 内毒素（Endotoxin） | Less than 1EU per μg by the LAL method. |
| 纯度（Purity） | > 95% as determined by Tris-Bis PAGE and HPLC |
| 活性（Activity） | ELISA Data: Immobilized Human CD3E&CD3G, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3E&CD3G Ab.1, hFc Tag with the EC50 of 1.22ug/ml determined by ELISA. ELISA Data: Immobilized Human CD3E&CD3G, hFc Tag at 1μg/ml (100μl/Well). Dose response curve for Anti-CD3E&CD3G Ab.2, mFc Tag with the EC50 of 20.7ng/ml determined by ELISA |
| 制剂（Formulation） | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| 重构方法（Reconstitution） | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230525
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