Recombinant Mouse AXL-Fc Chime

Generating NK Cell Receptor-Fc Chimera Proteins from 293T Cells and Considerations of Appropriate Glycosylation

cells. We present here (i) a detailed protocol for the production and purification of soluble recombinant NK cell receptors tagged with human IgG1-Fc (thus termed receptor-Fc chimera or receptor-Ig fusion protein) and (ii) a protocol for cell staining

Laboratory-Scale Purification of a Recombinant E-Cadherin-IgG Fc Fusion Protein That Provides a Cell Surface Matrix for Extended Culture and Efficient

and growth factors derived from mouse Engelbreth–Holm–Swarm �sarcoma cells. The complexity and lot-to-lot variation of Matrigel preparations has prompted the search for more defined substrates that can support pluripotent stem cell culture. A recombinant

Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes

with a Ca2+ -free medium or with trypsin/dispase. The latter facilitates the introduction of recombinant or trappable probes and microimaging studies of, for example, changes in cytosolic-free Ca2+ concentration or the dynamics of individual organelles