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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the C-terminus region of human JNK1 + JNK2. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX133806
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IHC-P
- 浓度:
0.44 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
JNK1 + JNK2
- 抗体英文名:
JNK1 + JNK2 antibody
- 抗体名:
JNK1 + JNK2 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Various whole cell and tissue extracts were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 + JNK2 antibody (GTX133806) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
JNK1 + JNK2 antibody detects JNK1 + JNK2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
JNK1 + JNK2 stained by JNK1 + JNK2 antibody (GTX133806) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK1 + JNK2 antibody (GTX133806) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Lin JT et al., Journal of Functional Foods 2018 47()
Yi Liu et al., Genes Dis 2021 (PMID:33997170)
Jau?Tien Lin et al., Journal of Food Biochemistry 2019 (Epub)
Huang C et al., Mol Ther Nucleic Acids 2019 (PMID:30826633)
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
results of phosphorylated proteins磷酸化的试剂盒中的抗体是经过大量筛选,选取了高特异性和低背景的抗体。p-P38MAPK抗体是基于P38的磷酸化Thr180到Tyr182这个位点多肽片段所制备。P-JNK抗体是基于人的Thr183和Tyr185.这个抗体可以同时识别P-JNK1和P-JNK2.我们在运用Cell Based Elisa P38和JNK两个试剂盒做检测的同时,又用传统的WB方式来验证这个结果。经过反复验证,都得出了同样的结果, Cell Based
相关专题 MAPK信号通路专题 c-Jun氨基末端激酶(c-Jun N-terminal kinase,JNK)又被称为应激活化蛋白激酶(stress-activated protein kinase,SAPK),是哺乳类细胞中MAPK的另一亚类。目前,从成熟人脑细胞中已克隆了10个JNK异构体,它们分别由JNK1、JNK2和JNK3基因编码[3],分子 量46 000的JNK1和分子量55 000的JNK2在各种组
基化等翻译后修饰可导致分子量增大,比如常见的CD家族蛋白糖基化修饰以后分子量都会增大许多;③蛋白质存在翻译后酶前体状态与激活剪切状态,可使分子量变小,比如MMP2蛋白,酶源与激活态大小分别是72kDa、63kDa;④蛋白质存在多个转录本由一个基因编码的形式,不同的转录本翻译得到的蛋白分子量不一样,比如JNK这个蛋白,本身分为JNK1,JNK2,JNK3三个亚型,三种亚型同源性较高,而且针对每个亚型还有不同分子量的转录本呈现;要想正确把握蛋白分量问题不仅仅要读说明书,还要关注这个蛋白的相关背景知识
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