- 询价
- 爱必信(absin)
- abs50009
- 上海
- 2025年07月16日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 英文名：
Annexin V-APC/PI apoptosis analysis kit
- 保质期：
Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.The validity period is 6 months
- 供应商：
爱必信(上海)生物科技有限公司
- 保存条件：
Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.The validity period is 6 months
- 规格：
25T/100T
| 规格： | 25T | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 100T | 产品价格： | 询价 |
| 描述 | Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Products List：Annexin V-APC、Propidium Iodide solution、Annexin V Binding Buffer、Apoptosis Positive Control Solution |
| 应用 | Each lot of this reagent is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used 5 µl/10^5 cells). Since applications vary, the appropriate dilutions must be determined for individual use. |
| 使用方法 | Suggested Staining Protocol A.Parameters regulation 1. Harvest cell(1×106 -3×106 cells), then sepatate the cells in two parts. Wash cells with cold PBS, then centrifuge the cells and disgard the supernatant. 2. Suspend one part of cells in 200μL 1× binding buffer, store at 4℃ for use. 3. Suspend the other part of cells in 500μL Apoptosis Positive Control Solution, and incubate for 10 minutes in room temperature. Wash cells with more than 3.0 mL cold PBS, blot the supernatant, then suspend the cells in 200μL 1×binding buffer. 4. Mix the two parts cells together, then separate the cells in three tubes, and add 100 μL of cells in each tube. 5. The first tube is Blank Control, the second one adds 5 μL of Annexin V-APC, and the third one adds 5 μL PI solution. 6. Gently vortex each tube and incubate for 5 minutes in room temperature, protected from light. 7. Before analyzing by flow cytometry, using the blank control and single dye sample to regulate voltage and compensation, as shown in Figure Parameters regulation. B.Sample detection 1. Dilute 3 mL 10×binding buffer with 27 mL distilled water for 10 tests. 2. Harvest cell（about 1×106cells per test）then wash with cold PBS. 3. Suspend cells in 1 mL 1× Binding Buffer, 300×g centrifugation for 10 minutes, then remove the Binding Buffer from the cell pellet. 4. Resuspend cells in 1 mL 1× Binding Buffer , adjust cell concentration to 1×1066cells/mL. 5. Add 100 μL of cells (1×105cells) to each labeled tube. 6. Add 5 μL of Annexin V-APC to appropriate tubes. 7. Gently vortex each tube and incubate for 10 minutes in room temperature, protected from light. 8. Add 5 μL PI solution incubation for 5min in room temperature, protected from light. 9. Add PBS to 500μL and vortex gently. 10. Analyze by flow cytometry in 1 hour. |
| 储存/保存方法 | Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.The validity period is 6 months. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验- 长链非编码RNA MALAT1通过抑制miR-144促进肝癌细胞增殖机制的研究段艺菲
青岛大学. 2020 Jun 16 ; 1
