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- 供应商:
北京赛百盛基因技术有限公司
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Cat. No.: UVSX-300 (for 300 μg)
Cat. No.: UVSX-1500 (for 1500 μg)
Component
T4 UvsX Recombinase (3 μg/μl, glycerol free)
Application
- DNA isothermal amplification
- RPA amplification
- Genetic testing
Heat Inactivation
60°C, 10min.
Storage Buffer
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 10% Trehalose, pH 7.5. The product does not contain glycerol and can be used to establish a freeze-drying system.
Storage
The minimum shelf life is 1 year at -20°C and 1 month at 4°C. Long-term storage at -80°C. Avoid repeated freezing and thawing.
Related: T4 UvsY Protein, Bsu DNA Polymerase (Large Fragment)
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文献和实验Assay for Human Rad51-Mediated DNA Displacement Loop Formation
replication forks, and for several other aspects of chromosome metabolism and maintenance. The homologous recombination reaction is mediated by the Rad51 recombinase. In the presence of ATP, Rad51 polymerizes on single-stranded DNA (ssDNA) to form
Construction of Gene-Targeting Vectors by Recombineering
should be phenotypically normal unless they are bred to a Cre-expressing transgenic line. Depending on the nature of the promoter controlling Cre recombinase expression (ubiquitous or tissue-specific), deletion of the lox P-flanked ("floxed") region can occur
A Comprehensive Guide to Sleeping Beauty–Based Somatic Transposon Mutagenesis in the Mouse
recombinase (http://nagy.mshri.on.ca/cre_new/index.php) Conditional or non‐conditional predisposing transgene (optional
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