T4 UvsX recombinase, derived from T4 phage, is a homolog of the RecA/Rad51 family. RecA/Rad51 recombinase family plays an important role in the repair of double-stranded DNA break and the restart of replication forks. T4 UvsX recombinase can anchor on single-stranded DNA together with other recombinase and activate it to scan for homologous sequences on other double-stranded DNA for further strand replacement. The enzyme has no nuclease activity.
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Cat. No.: UVSX-300 (for 300 μg)
Cat. No.: UVSX-1500 (for 1500 μg)
Component
T4 UvsX Recombinase (3 μg/μl, glycerol free)
Application
DNA isothermal amplification
RPA amplification
Genetic testing
Heat Inactivation
60°C, 10min.
Storage Buffer
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 10% Trehalose, pH 7.5. The product does not contain glycerol and can be used to establish a freeze-drying system.
Storage
The minimum shelf life is 1 year at -20°C and 1 month at 4°C. Long-term storage at -80°C. Avoid repeated freezing and thawing.