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- 库存:
大量
- 供应商:
北京百奥创新科技有限公司
- 规格:
1G;5G;250G;500G
CNBr活化琼脂糖磁珠
产品名称:CNBr活化琼脂糖磁珠英文名称:CNBr-activated SepFast
Product Introduction:
CNBr-activated agarose beads have a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to CNBr-activated agarose is a widely used, successful, and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.
This pre-activated SepFast beads can be readily employed to make various affinity chromatography media for both small scale and large scale purification applications. The application areas cover immobilization of proteins, peptides and nucleic acids.

Product Properties:
CNBr activated SepFast 4 High Flow (4HF) is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules.
The base matrix is reacted with cyanogen bromide (CNBr) to create active groups that react with the primary amine groups in the molecules to be immobilized. CNBr-activated SepFast media is supplied as a freeze-dried powder stabilized with additives.
Product Series:
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文献和实验S.L. Lim, H.W. Ng, M.A. Akwiditya, C.W. Ooi, E.S. Chan, K.L. Ho, W.S. Tan, G.K. Chua, B.T. Tey, Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography, Process Biochemistry, 2018, 69:208-215.
了一线技术支持常被问到的问题,希望这篇纯干货能帮助大家顺利升级打怪,早日驾驭 IP、co-IP。 IP、co-IP、pull-down 简介 IP(Immunoprecipitation),免疫沉淀:是利用固定在磁珠或琼脂糖树脂等基质上的特异性抗体对抗原进行小型亲和纯化的一种方法 Co-IP(co-Immunoprecipitation),免疫共沉淀: co-IP 是一种常用的鉴定蛋白-蛋白相互作用的方法,其基本实验流程和IP类似,通过使用诱饵蛋白(IP 中的抗原,bait protein)特异
技术丨化学发光免疫分析中羧基与Tosyl磁珠包被抗体方法及注意事项
羧基和甲苯磺酰基磁珠相关的抗体包被的方法以及影响因素。 化学发光操作步骤羧基和甲苯磺酰基(Tosyl)磁珠的对比 ※ 羧基磁珠反应原理 羧基和EDC反应活化,形成中间体,中间体和抗体的氨基反应,将抗体连接在磁珠表面。 ※ Tosyl磁珠反应原理 抗体根据自身的疏水性逐渐和疏水的Tosyl基团靠近,达到合适距离后,抗体所携带的氨基和Tosyl基团发生自然反应,将抗体连接在磁珠表面。 ※ 包被条件对比 _ Tosyl磁珠 羧基磁珠 活化
偶联链霉亲和素(SA)和抗体的实验方法及偶联实验过程中的一些注意事项。 一、羧基磁珠偶联链霉亲和素(SA)和抗体的原理 EDC通过与磁珠上的羧基进行反应,形成一种促发剂——不稳定的脲衍生物,NHS通过形成更稳定的酯而增强碳二亚胺交联产物的稳定性。交联过程中EDC、NHS不进入最终产物中,而是转变成水溶性的脲衍生物。 二、羧基磁珠偶联链霉亲和素(SA) 1. 偶联条件 1.1. 活化剂:EDC和NHS 1.2. 反应温度:37℃环境 1.3. 反应时间:






