琼脂糖*SIGMA*A6013-10g

说明

一般描述

Agarose is a polymer isolated from red seaweed, from the species Gelidium and Gracilaria, and it is commonly used for the separation of nucleic acids. Type I agarose is often utilized in molecular biology to make agarose gels for electrophoresis, a technique used to separate and analyze DNA or RNA fragments or proteins based on their size. Agarose may also be used in plant cell culture to create hydrogels, which are gel-like materials that provide a three-dimensional matrix. This provides a supportive and biocompatible environment for plant cells, this mimics physiological conditions and conducts experiments in vivo. They are versatile for various applications in plant biology, such as studying cell growth, tissue culture, and analyzing plant responses to different stimuli.

应用

Type 1 Agarose is commonly utilized for molecular biology:

for gel electrophoresis of DNA and RNA molecules[1][2]

for agarose overlay in cell culture, to measure antibacterial activity by radial diffusion[3]

for agarose overlay in cell culture for radio immunodiffusion assays to quantify antigens[4]

for agarose overlay in plaque assays to determine viral titer

to prepare agarose hydrogel that encapsulates a viscous media for 3D cell culture[5]

as an ingredient of human-tissue-like MRI phantoms[6]

for diagnostic to analyze patient samples for MRSA genome using pulsed-field gel electrophoresis[2]

特点和优势

Low EEO agarose is useful for routine analysis of nucleic acids

Suitable for electrophoresis: DNase & RNase free

Suitable for plant cell culture

Suitable for mammalian cell culture

分析说明

以下是与我们的琼脂糖相关的性质列表：

硫酸盐含量 - 用作纯度指标，因为硫酸盐是其中存在的主要离子基团。

凝胶强度 - 破裂凝胶所需施加的力。

胶凝点 - 琼脂糖水溶液在冷却时的胶凝温度。 琼脂糖溶液在液体-凝胶转变的过程中会表现出滞后现象 - 也就是说，它们的凝胶点与它们的熔点温度不同。

电内渗（EEO）- 液体通过凝胶的一种运动。琼脂糖凝胶中的阴离子基团被固定在基质上不能移动，但可解离的抗衡阳离子可以向基质中的阴极迁移，从而产生EEO。 由于生物聚合物的电泳运动通常是朝向阳极的，因此EEO会因为内部的对流而破坏分离。

 

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