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文献和实验Testing Rabbit Bleeds By Elisa
same amount of NaBH4 after 15''. Do a quick microfuge spin if there are too many bubbles. B. ELISA with Peptide conjugated to BSA Coat 10 ug/ml antigen (peptide conjugated to BSA) diluted in TBS (50 ul/well) ON at 4 deg C. Remove antigen
Immunofluorescence: Rabbit Anti-Murine RELMα
. Following fixation, the tissue was embedded in paraffin and cut into 5 μM sections. 1. Deparaffinize and rehydrate the tissue section. 2. Perform heat-induced antigen retrieval by boiling the tissue section in 10mM pH 6.0 citrate buffer
Antigen Design Sera Purification Tech Sheet
carrier protein. It is a good choice as a second carrier protein when verifying whether antibodies are specific for the peptide alone and not the carrier. Rabbit Serum Albumin (RSA) may be used when the antibody response to the carrier protein must be kept
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