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- abcam
- ab65866
- 2025年12月02日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
研卉生物
- 库存：
2周
- 英文名：
Native人IgE蛋白(Azide free)
- 规格：
100ug
ey features and details
- Expression system: Native
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 25.000 Eu/mg
- Suitable for: SDS-PAGE, IHC - Wholemount, WB, ELISA, ICC
-
产品名称
Native人IgE蛋白(Azide free)
参阅全部 IgE 蛋白酶 -
纯度
> 90 % SDS-PAGE.
Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum. -
内毒素水平
< 25.000 Eu/mg -
表达系统
Native -
Accession
-
蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
-
氨基酸序列 1
-
种属
Human -
额外的序列信息
Observed band of ~185kDa on SDS-PAGE
-
氨基酸序列 2
-
种属
Humanab65866 被引用在 8 文献中.
- Lindenburg L et al. Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins. Nucleic Acids Res 48:e63 (2020). PubMed: 32383757
- Vidal M et al. Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple Plasmodium falciparum antigens. J Immunol Methods N/A:N/A (2018). PubMed: 29397157
- Wan Z et al. Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing. PLoS One 13:e0191987 (2018). PubMed: 29389948
- Khramtsov P et al. Highly Stable Conjugates of Carbon Nanoparticles with DNA Aptamers. Langmuir N/A:N/A (2018). PubMed: 30089209
- Cui L et al. JSI-124 inhibits IgE production in an IgE B cell line. Biochem Biophys Res Commun 483:669-673 (2017). PubMed: 27988336
- van Beek N et al. Correlation of Serum Levels of IgE Autoantibodies Against BP180 With Bullous Pemphigoid Disease Activity. JAMA Dermatol N/A:N/A (2016). PubMed: 27829102
- Baltabekova AZh et al. SplitCore Technology Allows Efficient Production of Virus-Like Particles Presenting a Receptor-Contacting Epitope of Human IgE. Mol Biotechnol 57:746-55 (2015). PubMed: 25837568
- Goodman RE et al. Evaluation of endogenous allergens for the safety evaluation of genetically engineered food crops: review of potential risks, test methods, examples and relevance. J Agric Food Chem 61:8317-32 (2013). SDS-PAGE . PubMed: 23848840
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- 作者
- 内容
- 询问日期
文献和实验自由基是指那些在原子核外层轨道上具有不成对电子的分子或原子基团。所谓未成对电子,就是指那些在原子或分子轨道中未与其它电子配对而独占一个轨道的电子。如AB两个原子各提供一个电子通过共价键形成一个分子A∶B, 这两个电子是配对的。如果在化学反应中发生了均裂, A和B各带走一个电子,它们就是未成对电子。 A∶B → A· ·B A·和·B就称为自由基。 自由基的种类很多，活性最强的是氧中心自由基，简称氧自由基，包括·02 （超氧自由基）、·OH
Structure-Based Ab Initio Prediction of Transcription FactorBinding Sites
We present an all-atom molecular modeling method that can predict the binding specificity of a transcription factor based on its 3D structure, with no further information required. We use molecular dynamics and free energy calculations
Phycoerythrin conjugation protocol
concentrator (Centricon 30 or 100 are fine) Apply to top of separation column. Elute with PBS. Pump column at approximately 0.3 ml/min. Order of elution is conjugate first (MW ~400,000) then free PE (MW ~240,000) then free Ab (MW ~150,000
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