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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG
- 形态:
液体
- 保存条件:
-20℃
- 克隆性:
多克隆
- 标记物:
无
- 适应物种:
HMR,Rabbit
- 保质期:
一年
- 抗原来源:
兔
- 供应商:
万类生物
- 宿主:
兔
- 应用范围:
WB,IHC
- 浓度:
>1 mg/ml
- 靶点:
PARP/ cleaved-PARP
- 抗体英文名:
PARP/cleaved-PARP antibody
- 抗体名:
PARP/ cleaved-PARP 抗体
- 规格:
50μl(>50μg)
产品概述
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase comprise a superfamily of enzymes that functionally modify histones and other nuclear proteins, thereby preventing cell death.This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.PARP proteins are implicated in a variety of diseases, including cancer, neurodegenerative and inflammatory disorders.
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文献和实验【求助】请问western blot做凋亡检测时,识别caspase-3酶原和激活的caspase-3的抗体是相同的吗?
lsdcfhyj 以及检测PARP的裂解前后蛋白水平用的抗体是否都是一样的? edwardellen 有两者都识别的,也有专门识别剪切体不识别酶原的,用过cell signal的,两种都很好用。 PARP也有可以同时识别全长和剪切体的抗体。 magichunter 检测caspase-3和激活型的caspase-3分别需要两种不同的抗体,一般CST公司卖的分别包括:cleaved
Caspase-3(CPP-32,Apoptain,Yama,SCA-1),半胱天冬酶家族成员之一,是凋亡的关键执行者,对许多关键的蛋白起着部分或全部的裂解作用,如核酶多聚(ADP-ribose)聚合酶(PARP)[1] 及众多细胞骨架相关的蛋白、激酶以及 Bcl-2 家族中凋亡相关的蛋白等[2] 。Caspase-3 在肺、脾脏、心脏、肝脏、肾脏等中广泛表达,在大脑和骨骼肌中表达量稍低,在睾丸中表达量最低。同时在许多细胞系中都有发现 caspase-3 的表达,其中免疫相关的细胞中表达量最高
Phenotypic Characterization of Parp-1 and Parp-2 Deficient Mice and Cells
, whereas a specific female lethality related to X chromosome instability is associated with the Parp-1+/− Parp-2−/− genotype. Finally, recent research discovered emerging unique functions of Parp-2 in physiological processes including spermatogenesis, T-cell
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