- ￥2630
- CUSABIO
- 武汉
- CSB-PA270960
- 2025年07月09日
- ELISA, WB, IF, IP
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide corresponding to the amino terminus of histone H4 in which Arg3 is mono-methylated.
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
-20°C
- 克隆性：
多克隆
- 标记物：
Non-conjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12个月
- 抗原来源：
Human
- 目录编号：
CSB-PA270960
- 级别：
多克隆抗体
- 库存：
充足
- 供应商：
武汉益普生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
ELISA, WB, IF, IP
- 靶点：
HIST3H3
- 抗体英文名：
Histone H4R3me1 Antibody
- 规格：
100μl
| 产品名称 | Histone H4R3me1 Antibody |
| 货号 | CSB-PA270960 |
| 别名 | H4; H4/n; H4F2; H4FN; FO108; HIST2H4 |
| 抗体类型 | Polyclonal Antibody |
| 克隆类型 | Polyclonal |
| Uniprot No. | GeneID:8290SwissProt:Q16695 |
| 基因名 | HIST3H3 |
| 免疫原种属 | Human |
| 免疫原 | A synthetic peptide corresponding to the amino terminus of histone H4 in which Arg3 is mono-methylated. |
| 宿主 | Rabbit |
| 反应种属 | Human, Mouse, Rat |
| 应用范围 | ELISA, WB, IF, IP |
| 抗体亚型 | IgG |
| 标记方式 | Non-conjugated |
| 产品提供形式 | liquid |
| 纯化方式 | Antigen Affinity Purified |
| 研究领域 | Epigenetics and Nuclear Signaling |
| 保存缓冲液 | Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| 储存条件 | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
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文献和实验PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
Chromatin Immunoprecipitation Protocol for Histone Modification
. This has been very successful when using antibodies that detect site specific histone lysine methylation (Peters et al . 2003, Martens et al . 2005). However, for each different antibody the optimal concentration should be tested and calibrated. The stringency of washing
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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