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文献和实验Prior to the initiation of experiment: Obtain IACUC approval Sterilize surgical instruments and Q-tips I. Harvest Donor Tissue Euthanize donor mouse (in accordance with institution's SOP) Wet hair
Experimental animal models are available for the development of new treatment. Murine animal models have particular advantages for comparative study to evaluate the efficacy and safety of different treatment modalities because many mice
Isolation of murine splenocytes
attached (Protocol A only) frosted-end glass slides (x 2) (Protocol B only) 50ml conical tube (Protocol B only) Reagents DMEM-10 (about 20mL) 1L DMEM (with 4.5g/L glucose, L-glutamine, sodium pyruvate
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