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文献和实验Prion Protein as Copper-Binding Protein at the Synapse
(1 ). The glial fibrillary acidic protein was one of the first possible binding partners to be described (2 ) followed by Bcl-2 (3 ,4 ), molecular chaperones (5) , amyloid precursor-like protein 1 (6 ), the 37-kDa laminin receptor (7 ) and a 66-kDa membrane
or the glial cell marker glial fibrillary acidic protein, the cells expressing PDE9 mRNA were further characterized. Double-labeling experiments revealed that PDE9 was predominantly expressed in neurons.
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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