Atherosclerosis measurement in male WT → Ldlr KO and Lpcat3 KO → Ldlr KO mice. (A) Aortic arches with atherosclerotic plaques (white areas). (B) Aortic root assay for lesion areas after H&E staining. Six alternate sections (10 μm thick) sliced from paraffin-fixed aortic root tissues of each transplanted mouse were used for the analysis. (C) En face aortic plaque analysis after Oil Red O staining. (D) Quantitative display of root assay. (E) Quantitative display of en face assay. Red arrows indicate lesion area. Values are mean ± SD. N = 8–9.