Data determined by nanoparticle tracking analysis (NTA). Each curve represents the average of 3 technical replicate measurements for each exosome isolation method and biofluid triplicate experiment. (PM = Precipitation Method). Figure taken and adapted from (Martins, TS et al., 2018)
Data determined by NTA and EXOCET. Serum sample has been analysed for qualitative comparison between Exo-spin™ and precipitation methods.
订购信息:
货号
描述
EX02-8
Exo-spin™ blood, 8 columns, 2 ml buffer
EX02-25
Exo-spin™ blood, 24 columns, 25 ml buffer
EX02-50
Exo-spin™ blood, 48 columns, 30 ml buffer
下游应用:建议和内容
RNA analysis:
Wang L., Wang C., Jia X. and Yu J. (2018). Circulating Exosomal miR-17 Inhibits the Induction of Regulatory T Cells via Suppressing TGFBR II Expression in Rheumatoid Arthritis. Cellular Physiology and Biochemistry; 50:1754–1763.
Emanueli C., Shearn AI., Laftah A., Fiorentino F., Reeves BC., Beltrami C., Mumford A., Clayton A., Gurney M., Shantikumar S. and Angelini G. (2016). Coronary Artery-Bypass-Graft Surgery Increases the Plasma Concentration of Exosomes Carrying a Cargo of Cardiac MicroRNAs: An Example of Exosome Trafficking Out of the Human Heart with Potential for Cardiac Biomarker Discovery.PLoS One29;11(4):e0154274.
Mass Spectrometry:
Menezes-Neto A., Fidalgo Sáez MJ., Lozano-Ramos I., Segui-Barber J., Martin-Jaular L., Estanyol Ullate JM., Fernandez-Becerra C., Borrás FE., and del Portillo HA. (2015). Size-exclusion chromatography as a stand-alone methodology identifies novel markers in mass spectrometry analyses of plasma-derived vesicles from healthy individuals.Journal of Extracellular Vesicles; 4: 10.3402/jev.v4.27378.
Functional study:
Sheller-Miller S, Trivedi J, Yellon SM, and Menon R. (2019). Exosomes Cause Preterm Birth in Mice: Evidence for Paracrine Signaling in Pregnancy. Scientific Reports24;9(1):608.