中文名称:人子宫内膜癌细胞 Ishikawa人子宫内膜癌细胞/Ishikawa细胞 英文名称:Ishikawa cell 品牌:accegen 产品简介:Ishikawa人子宫内膜癌细胞/Ishikawa细胞。Ishikawa细胞系是从一名39岁女性的子宫内膜腺癌中建立的。该细胞在无胸腺裸鼠中诱导分化良好的腺癌。雌激素和孕激素受体在细胞培养和诱导肿瘤中均得到证实。据报道,石川产生促肾上腺皮质激素释放激素,胎盘碱性磷酸酶和绒毛膜促性腺激素,并对类固醇激素有反应。 石川细胞是一种人子宫内膜腺癌细胞系,来源于一名 39 岁的子宫内膜腺癌女性。这些细胞表现出马赛克状的单层生长模式,并倾向于形成绒毛。染色体分析揭示了模态染色体数目的二倍体范围。当移植到无胸腺裸鼠体内时,诱导的肿瘤表现出与原始人类肿瘤相似的分化良好的腺癌特征。在体外培养的细胞和诱导的肿瘤中都观察到雌激素受体和孕激素受体的存在。在暴露于具有不同雌激素活性的化学物质后,石川细胞可以表现出生物学上显着的雌激素反应。 Ishikawa cells are a human endometrial adenocarcinoma cell line derived from a 39-year-old woman with endometrial adenocarcinoma. These cells exhibit a mosaic-like monolayer growth pattern and tend to form piles. Chromosomal analysis revealed a diploid range of modal chromosomal numbers. When transplanted into athymic nude mice, the induced tumor exhibited well-differentiated adenocarcinoma characteristics similar to the original human tumor. The presence of estrogen receptor and progesterone receptor was observed both in the in vitro cultured cells and the induced tumor. Following exposure to chemicals with diverse estrogenic activity, Ishikawa cells can demonstrate a biologically significant estrogenic response. 为什么选择AcceGen的Ishikawa? AcceGen 的 Ishikawa 细胞因其高活力和高质量而成为选择。这些细胞保证是无菌的,并由专业操作员在最佳标准下进行孵育和冷冻保存过程。AcceGen 确保严格的质量控制措施,使他们的 Ishikawa 细胞成为各种研究应用的可靠选择。 Why choose Ishikawa from AcceGen? Ishikawa cells from AcceGen are the preferred choice due to their high viability and quality. These cells are guaranteed to be sterile and have undergone incubation and cryopreservation processes carried out by professional operators under optimal standards. AcceGen ensures rigorous quality control measures, making their Ishikawa cells a reliable option for various research applications. 产品类型:人子宫癌症细胞系 细胞培养流程: 1、培养前准备 (1)培养器具:推荐使用25cm2培养瓶或60mm培养皿(或更小)。 (2)培养基:准备适合培养Ishikawa人子宫内膜癌细胞/Ishikawa细胞的培养基,建议用量5 mL(或更少)。 2、细胞解冻 将包装中的冻存管取出后立即放在不高于37℃的水中,并在2分钟内摇晃解冻。 3、细胞复苏 (1)取10ml培养基置于离心管中,用70%酒精或阳离子洗涤剂渗透纱布消毒冻存管,用消毒纱布包裹管身,拧开管盖后,将细胞悬浮液转移到离心管中; (2)1000 rpm离心3分钟,弃掉上清液; (3)将细胞重新悬浮在5ml上述培养基中(不清洗细胞)。在25cm2的培养瓶或60mm的培养皿中进行培养。 4、细胞传代 在传代之前,确保细胞增殖,因为过度稀释可能导致细胞死亡,尤其是血细胞和淋巴细胞。 5、细胞冻存 待Ishikawa人子宫内膜癌细胞/Ishikawa细胞生长状态良好时,尽早进行细胞冻存。
ISHIKAWA
Product Name
Ishikawa人子宫内膜癌细胞/Ishikawa细胞
Cat.No
ABC-TC1320
Species
Human
Size/Quantity
1 vial
Shipping Info
Dry Ice
Storage
Liquid Nitrogen
Growth Properties
Epithelial-like
Recommended Medium And Supplement
MEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Foetal Bovine Serum (FBS)