- ￥34602
- Absolute Antibody
- Ab00667-13.0-BT
- 英国
- 2025年07月14日
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文献和实验Inhibition of Glycogen Synthase Kinase-3 Methods in Molecular Biology v468. chapter 5 Abstract There are two homologous forms of glycogen synthase kinase (GSK)-3, GSK-3a and GSK-3b, whichplay overlapping roles in the regulation of Wnt
7.5, 150 mM NaCl, and1% (v/v) Triton X-100. 7. Suitable SDS-PAGE and transfer setup as in Section 2.4.1 . 8. Protein Sample Loading Dye (4×) as in Section 2.4.1 . 9. Nitrocellulose or PVDF membrane. 10. Mouse anti-β-catenin antibody (available
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Ususally 1:1000 (v/v) in PBS. Wash in PBS 2' x3. Equilibrate slides in Buffer 1, RT x 5'. Incubate slides in Blocking Buffer, RT x 30' Incubate with anti-Dig-AP conjugate (1:2000 in Blocking buffer.), 2 hrs. RT, or o.n. at 4ºC.
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