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文献和实验Methods to Assess Inhibition of Adipocyte Differentiation by SV40 Large T Antigen
. Preadipocytes transformed by a variety of oncogenic proteins, such as the Simian virus 40 (SV40) large-tumor antigen (SVLT) or activated ras oncogene (1 –6 ) and others, fail to differentiate into adipocytes. However, the transformation and differentiation
SV40 large T antigen (T-ag) is an oncoprotein that induces transformation of cells through the binding and inactivation of the p53 tumor suppressor protein and the retinoblastoma family of proteins. This chapter focuses on the interaction
Biology of p53 and SV40 Large T Association
p53 was discovered because of its capacity to bind to SV40 large T antigen (SVLT) in transformed cell lines (1 ,2 ). It is a phosphorylated, 393 amino acid protein, with five blocks of sequences that are highly conserved
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