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- 2025年07月03日
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文献和实验Assembly of Nucleosomal Templates by Salt Dialysis
Basic Protocol 2: Assembly of High Concentration of Mononucleosomes by Gradient Salt Dialysis Materials Unlabeled DNA fragment
are often part of the normal nasopharyngeal flora, especially in young children. Pneumococcal colonization is an important risk factor: children colonized with S. pneumoniae more often develop acute otitis media than children who are not colonized (2 -5 ).
Polymerase Chain Reaction (PCR) to Amplify rRNA Gene Fragment
Polymerase Chain Reaction (PCR) to Amplify rRNA Gene Fragment Prepare sufficient master mix for both partners (45 mL/50 mL reaction) 10 mL 10x PCR buffer 10 mL 2.5 mM dNTPs (0.25 mM final concentration
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