Crystal Chem90105完整胰岛素原ELISA试剂

【讨论】抑制免疫到底是好还是坏?

cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2

Performing Bronchoalveolar Lavage in the Mouse

and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain

[转贴]包涵体纯化复性

系统照相。 超声波裂解细菌 渗透休克细菌沉淀加1/10培养体积20 mM Tris-HCl（pH 7.5，0℃）重悬，冰浴，超声波裂解，20%工作选择，脉冲粉碎1 min，然后13000 r/min离心5 min，-20℃保存上清、细菌沉淀。上清进行TCA沉淀，处理同上。沉淀用Protein Extraction Reagent（Ni-NTAHi Bind Purification Kit BugBuster. 提供 ）按 Novagen公司《pET System Manual》提供方法反复处理