Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
SWISS: P0DTC9
Gene ID: 43740575
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Sample: Lane 1: SARS-CoV-2 N protein(WT) at 10ng Lane 2: SARS-CoV-2 N protein(Q9H, P67S, P80R, P151L, S183Y) at 2ng Lane 3: SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y,H145Y, R203K, G204R, T205I, S235F) at 2ng Lane 4: SARS-CoV-2 N protein(Del204 & Del215) at 2ng Lane 5: SARS-CoV-2 N protein(R203M & D377Y) at 2ng Primary: Anti- SARS-CoV-2 N protein at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD