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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
- 适应物种:
Human, Mouse, Rat
- 保质期:
12个月
- 库存:
现货
- 宿主:
Mouse
- 应用范围:
WB
- 靶点:
14-3-3 eta
- 规格:
50μL/100μL
| 规格: | 50μL | 产品价格: | ¥1400.0 |
|---|---|---|---|
| 规格: | 100μL | 产品价格: | ¥2400.0 |
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文献和实验7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
-0031 , or Purified, Cat. No. 14-0031 ) Complete RPMI-1640 Sterile single-cell suspension of mouse spleen or lymph nodes 96-well flat-bottom microtiter plates with lids (Costar Cat. No. 3596) MTT Buffer MTT Lysing Solution
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