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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C. Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
- 适应物种:
Human
- 保质期:
12个月
- 库存:
现货
- 宿主:
Rabbit
- 应用范围:
WB,IHC,ICC/IF
- 靶点:
Kinesin Heavy Chain 2
- 规格:
20μL/50μL/100μL
| 规格: | 20μL | 产品价格: | ¥600.0 |
|---|---|---|---|
| 规格: | 50μL | 产品价格: | ¥1000.0 |
| 规格: | 100μL | 产品价格: | ¥1800.0 |
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文献和实验Generation of Rabbit Immune Libraries
for the combinatorial cloning of the variable heavy and light chain immunoglobulin repertoire of rabbits for the subsequent phage display of rabbit scFv antibody fragments is described.
by staining in simple IHC the relevant heterologous primary antibodies. E.g. many goat anti mouse IgG1 heavy chain do stain rat IgG2a and IgG2b . Procedure: First stain HRP Developing solution Second stain AP
(e.g. Luciferase); then a secondary antibody conjugated to a flourochrome or enzyme is bound to the heavy chain constant region of the primary antibody with specificity to the species of origin of the primary (e.g. anti-rabbit conjugated to Rhodamine
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