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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.
- 适应物种:
Transfected
- 保质期:
12个月
- 库存:
现货
- 宿主:
Mouse
- 应用范围:
WB
- 靶点:
T7 Tag
- 规格:
50μL/100μL
| 规格: | 50μL | 产品价格: | ¥1400.0 |
|---|---|---|---|
| 规格: | 100μL | 产品价格: | ¥2400.0 |
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文献和实验WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
X transcription buffer (Stratagene) 2 µl labelling mix 1 µl RNAGUARD (Pharmacia) 1 µl RNA polymerase (SP6, T3 or T7) Add water to 20 µl Incubate for 2 hours at 37 °C. Run on 1% agarose gel (1.5µl
Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
WHOLE MOUNT IN SITU HYBRIDIZATION.Mouse Embryos
expressed in the anterior primitive endoderm of the mouse gastrula" Mechanism of Development, in press - 1997. Modified from the protocol of Harland (1991) and Current Protocols in Molecular Biology. Preparation of the probe.
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