PARP1 (10C2) Mouse mAb

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed/17177976, PubMed/18172500, PubMed/19344625, PubMed/19661379, PubMed/23230272). Mediates the poly(ADP-ribosyl)ation of APLF and CHFR (PubMed/17396150). Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (PubMed/17177976). Required for PARP9 and DTX3L recruitment to DNA damage sites (PubMed/23230272). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed/23230272). Mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity (PubMed/28190768). Mediates the poly(ADP-ribosyl)ation of histones in a HPF1-dependent manner (PubMed/27067600). Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5 (PubMed/27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed/27257257).