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PARP1 (10C2) Mouse mAb

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  • ¥1400 - 2400
  • 戴格
  • db6467
  • 2025年11月17日
  • WB,IHC-P
  • Mouse
  • Human, Mouse, Rat
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Store at -20°C. Supplied in PBS, 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Stable for 12 months from date of receipt.

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      12个月

    • 库存

      现货

    • 宿主

      Mouse

    • 应用范围

      WB,IHC-P

    • 靶点

      PARP1

    • 规格

      50μL/100μL

    规格:50μL产品价格:¥1400.0
    规格:100μL产品价格:¥2400.0
    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed/17177976, PubMed/18172500, PubMed/19344625, PubMed/19661379, PubMed/23230272). Mediates the poly(ADP-ribosyl)ation of APLF and CHFR (PubMed/17396150). Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (PubMed/17177976). Required for PARP9 and DTX3L recruitment to DNA damage sites (PubMed/23230272). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed/23230272). Mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity (PubMed/28190768). Mediates the poly(ADP-ribosyl)ation of histones in a HPF1-dependent manner (PubMed/27067600). Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5 (PubMed/27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed/27257257).

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    图标文献和实验
    相关实验
    • Purification of mAb (IgG)

      (adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10

    • Purification of mAb (IgG)

        Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers

    • T-Cell Activation Using mAb to CD3

      One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse

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    PARP1 (10C2) Mouse mAb
    ¥1400 - 2400