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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Recombinant Human Siglec-15/CD33L3(Mammalian,C-6His)推荐
- 库存：
2
- 供应商：
研卉生物
- 规格：
50ug
Recombinant Human Siglec-15/CD33L3 (CW37)
产品说明(Description)
Recombinant Human Siglec-15 is produced by our Mammalian expression system and the target gene encoding Phe20-Thr263 is expressed with a 6His tag at the C-terminus.
Accession #: Q6ZMC9
Known as: Sialic acid-binding Ig-like lectin 15; Siglec-15; CD33 antigen-like 3; CD33L3
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of PBS, 150mMNacl, 0.3%chaps, 5% Trehalose, pH 7.4.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
| Bioactivity-Elisa: |
Bioactivity-BLI: |
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| Immobilized Human Siglec-15-His (Cat#CW37) at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 48.5 ng/ml. |
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Loaded Anti-Human Siglec15 mAb-mFc(Cat#NC007) on AMQ Biosensor, can bind Human Siglec-15-His(Cat#CW37) with an affinity constant of 1.47 nM as determined in BLI assay. |
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
背景(Background)
Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO?-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.
电泳(SDS-PAGE)
参考文献(Reference)
Magnetic field-aligned Fe 3 O 4-coated silver magnetoplasmonic nanochain with enhanced sensitivity for detection of Siglec-15[J]. Xing Huang. et al. Biosensors and Bioelectronics. 2021.
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