- ¥520
- 江蓝纯
- M1001060
- 2025年07月16日
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- 库存：
99
- 供应商：
江蓝纯
- 规格：
100ul
| 英文名称 | anti-Flag Tag Mouse Monoclonal antibody |
|---|---|
| 应用 | WB 1:2500 IP 1:100 IF:1:500 Optimal dilutions should be determined by the end user. |
| 浓度 | 1mg/ml |
| 保存 | Store at -20°C. Do not aliquot the antibody. |
| 储存液 | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol |
| 纯化方法 | affinity purified by Protein A |
| 亚型 | IgG |
| 性状 | Liquid |
| 克隆类型 | monoclonal |
| 来源 | Mouse |
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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