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- 库存:
99
- 供应商:
爱必信(上海)生物科技有限公司
- 规格:
10ug/50ug/1mg
| 规格: | 10ug | 产品价格: | ¥1109.0 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥2877.0 |
| 规格: | 1mg | 产品价格: | ¥37422.0 |
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试剂描述: 产品名称:rRLeptin tA 产品别名:Recombinant Rat Leptin Triple Antagonist;重组大鼠瘦素三倍体拮抗剂 CAS号: 英文别名:rRLeptin tA 分子量: 分子式: 纯度:99.0% 外观:见爱必信官网 保存方法:Lyophilized Leptin-Antagonist Triple Mutant Rat Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP-tA mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent free 描述: Leptin Antagonist Triple Mutant Rat Recombinant is a singly non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A mutant. Leptin Antagonist Triple Mutant Rat Recombinant was purified by proprietary chromatographic techniques. 应用:
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文献和实验TA Cloning exploits the terminal transferase activity of some DNA polymerases such as Taq polymerase. This enzyme adds a single, 3'-A overhang to each end of the PCR product. This makes it possible to clone this PCR product directly
Instructions Modified for Simpson Lab 1.PCR: Perform a standard PCR reaction using taq
This protocol uses Promega''s pGEM-T kit (#A3600). PCR For TA cloning, it is optimal if the PCR primers have G''s at the 5'' end as this will maximize the probability of Taq polymerase adding the terminal A overhang
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