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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
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- 抗体英文名:
,PRKAR2A; PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit
- 靶点:
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- 浓度:
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- 应用范围:
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- 宿主:
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- 适应物种:
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- 保质期:
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- 抗原来源:
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- 目录编号:
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- 级别:
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- 库存:
99
- 供应商:
爱必信(上海)生物科技有限公司
- 标记物:
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- 克隆性:
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- 保存条件:
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
- 形态:
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- 亚型:
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- 免疫原:
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- 规格:
100ul
| 概述 | |
| 别名 | PRKAR2A; PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit |
| 宿主 | Rabbit |
| 反应种属 | Human,Mouse,Rat,Pig |
| 应用 | WB: 1/500-1/1000, IHC: 1/50-1/100, IF: 1/50-1/200, IP: 1/20 |
| 分子量 | Detects a band of approximately 51 kD(Predicted molecular weight: 46 kD) Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 | A synthesized peptide derived from human Phospho-PKA R2 (S99) |
| 性能 | |
| 形式 | Polyclonal Antibody |
| 纯化方法 | Affinity Chromatography |
| 同种型 | IgG |
| 储存/保存方法 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 存储溶液 | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 修饰 | Phosphorylated |
| 靶标 | |
| 背景说明 | Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. |
| UniProt | P13861 |
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文献和实验secondary antibody review -- data from 99 publications
IgG Alexa Fluor 488 1:250 Molecular Probes 10 goat Cy3 immunocytochemistry detect NMDA receptors in HEK293 cells Jackson ImmunoResearch Laboratories 10 FITC immunocytochemistry 1:100 detect phospho-CREB in human colonic
:使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
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