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- 爱必信(absin)
- abs158146
- 上海
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
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- 抗体英文名：
,BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
- 靶点：
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- 浓度：
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- 应用范围：
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- 宿主：
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- 适应物种：
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- 保质期：
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- 抗原来源：
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- 目录编号：
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- 级别：
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- 库存：
99
- 供应商：
爱必信(上海)生物科技有限公司
- 标记物：
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- 克隆性：
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- 保存条件：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
- 形态：
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- 亚型：
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- 免疫原：
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- 规格：
100ul
| 概述 | |
| 别名 | BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1 |
| 宿主 | Mouse |
| 反应种属 | Human,Mouse |
| 应用 | WB: 1/500-1/1000 |
| 分子量 | Detects a band of approximately 87 kD(Predicted molecular weight: 84 kD) Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 | Purified recombinant human B Raf protein fragments expressed in E.coli. |
| 性能 | |
| 形式 | Monoclonal Antibody |
| 纯化方法 | Affinity Purified |
| 克隆号 | 4E1 |
| 同种型 | IgG2a |
| 储存/保存方法 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 存储溶液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| 修饰 | Unmodified |
| 靶标 | |
| 背景说明 | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. |
| UniProt | P15056 |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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