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- 爱必信(absin)
- abs158120
- 上海
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
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- 抗体英文名：
,CHD3; Chromodomain-helicase-DNA-binding protein 3; CHD-3; ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase; hZFH
- 靶点：
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- 浓度：
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- 应用范围：
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- 宿主：
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- 适应物种：
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- 保质期：
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- 抗原来源：
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- 目录编号：
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- 级别：
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- 库存：
99
- 供应商：
爱必信(上海)生物科技有限公司
- 标记物：
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- 克隆性：
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- 保存条件：
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
- 形态：
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- 亚型：
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- 免疫原：
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- 规格：
100ul
| 概述 | |
| 别名 | CHD3; Chromodomain-helicase-DNA-binding protein 3; CHD-3; ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase; hZFH |
| 宿主 | Mouse |
| 反应种属 | Human,Mouse,Rat |
| 应用 | WB: 1/500-1/1000, IF: 1/50-1/200, IP: 1/20 |
| 分子量 | Detects a band of approximately 260 kD(Predicted molecular weight: 227 kD) Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 | Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli. |
| 性能 | |
| 形式 | Monoclonal Antibody |
| 纯化方法 | Affinity purified |
| 克隆号 | 7F6 |
| 同种型 | IgG1 |
| 储存/保存方法 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 存储溶液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| 修饰 | Unmodified |
| 靶标 | |
| 背景说明 | Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity. |
| UniProt | Q12873 |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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