- ￥1500
- 爱必信(absin)
- abs113348
- 上海
- 2025年07月12日
- WB~~1:1000
- Rabbit
- -
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
VSV-g Tag抗体
- 抗体英文名：
Rabbit anti-VSV-g Tag Polyclonal Antibody
- 靶点：
-
- 浓度：
-
- 应用范围：
WB~~1:1000
- 宿主：
Rabbit
- 适应物种：
-
- 保质期：
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
- 抗原来源：
-
- 目录编号：
-
- 级别：
-
- 库存：
现货
- 供应商：
爱必信(上海)生物科技有限公司
- 标记物：
-
- 克隆性：
-
- 保存条件：
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
- 形态：
液态
- 亚型：
-
- 免疫原：
-
- 规格：
50ul
| 别名 | VSV-g Tag抗体 |
| 宿主 | Rabbit |
| 特异性 | KLH conjugated synthetic peptide encoding VSV-g tag (CYTDIEMNRLGK) was used as antigen. |
| 应用 | WB~~1:1000 |
| 类型 | Polyclonal Antibody |
| 同种型 | Rabbit Ig |
| 储存/保存方法 | Maintain refrigerated at 2-8°C for up to 2 weeks. For long time storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| 存储溶液 | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| 研究领域 | Tags |
| 背景说明 | Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. VSV-g is an epitope tag composed of an 11 residue peptide, YTDIEMNRLGK, derived from the Vesicular Stomatitis viral glycoprotein. Abgent's anti-VSV-g polyclonal antibody provides a simple solution to detect the expression of a VSV-g-tagged protein in cells. |
| 参考文献 | Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13329-34. J Virol. 1998 Jan;72(1):428-35. J Virol. 2002 Dec;76(23):12300-11. |
Western blot analysis of anti-Tag-VSV-G Pab (Cat. #AP1016a) in 12tag cell line lysates (35ug/lane). Tag-VSV-G(arrow) was detected using the purified Pab.
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文献和实验- The EF-Hand Protein CALML6 Suppresses Antiviral Innate Immunity by Impairing IRF3 Dimerization Wang Z,Sheng C,Yao C,Chen H,Wang D,Chen S
Cell Rep. 2019 Jan 29 ; 5
影响因子： 7.815
干货 | CUT and Tag 让 ChIP-Seq更简单高效！
说明书推荐的反应体系进行 CUT&Tag 实验；其中，pG-Tn5 转座子使用的终浓度为 0.04μM ，一抗为 H3K27me3（CST，#9733），二抗为 Goat anti Rabbit（Bioworld，#BS13271）。 A、文库峰型检测 使用 Agilent 2100 Bioanalyzer 进行文库分布检测。 图 2. 使用 TD901 构建 100 cells CUT&Tag 文库峰型图 图 3. 使用 TD901 构建 10,000 cells CUT&Tag
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
2c + ++ Sources of information: Akerstrom, B., Brodin, T., Reis, K., and Bjorck, L. 1985. Protein G: A powerful tool for binding and detection of monoclonal and polyclonal antibodies. J. Immunol. 135:2589-2592. Akerstrom, B. and Bjorck, L. 1986
Polyclonal Antibody Production
For injection of two rabbits, your antigen should be in 1 mL of a buffer such as PBS or Tris that contains no chemicals harmful to the rabbit. 100 µg/rabbit of antigen is best, but you can use less and get good results.
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