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- abs101405
- 上海
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
FSTL3抗体
- 抗体英文名：
Follistatin-related protein 3, Follistatin-like protein 3, Follistatin-related gene protein, FSTL3, FLRG,FSTL3,FLRG
- 靶点：
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- 浓度：
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- 应用范围：
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- 宿主：
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- 适应物种：
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- 保质期：
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- 抗原来源：
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- 目录编号：
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- 级别：
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- 库存：
现货
- 供应商：
爱必信(上海)生物科技有限公司
- 标记物：
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- 克隆性：
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- 保存条件：
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
- 形态：
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- 亚型：
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- 免疫原：
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- 规格：
50ul
| 描述 | Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| 别名 | FSTL3抗体;Follistatin-related protein 3, Follistatin-like protein 3, Follistatin-related gene protein, FSTL3, FLRG,FSTL3,FLRG |
| 宿主 | Rabbit |
| 特异性 | This FSTL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-258 amino acids from the C-terminal region of human FSTL3. |
| 反应种属 | Human |
| 应用 | IHC-P~~1:25 WB~~1:1000 |
| 分子量 | Predicted molecular weight: 28kD Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 类型 | Polyclonal Antibody |
| 同种型 | Rabbit Ig |
| 储存/保存方法 | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| 存储溶液 | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| 研究领域 | Signal Transduction |
| 背景说明 | Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10. |
| 细胞定位 | Isoform 1: Secreted. |
| UniProt | O95633 |
| 参考文献 | Miron, P., et al. Prenat. Diagn. 30(3):224-228(2010) Thadhani, R., et al. Diabetes Care 33(3):664-669(2010) Bloise, E., et al. BMC Cancer 9, 320 (2009) : Stamler, R., et al. J. Biol. Chem. 283(47):32831-32838(2008) Lara-Pezzi, E., et al. Endocrinology 149(11):5822-5827(2008) |
Anti-FSTL3 Antibody (C-term) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-FSTL3 Antibody (C-term) at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
FSTL3 Antibody (C-term) (Cat. #abs101405) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FSTL3 antibody detected the FSTL3 protein (arrow).
FSTL3 Antibody (C-term) (Cat. #abs101405)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FSTL3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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文献和实验- Follistatin-Like 3 Correlates With Lymph Node Metastasis and Serves as a Biomarker of Extracellular Matrix Remodeling in Colorectal CancerChao Yang, Fengyu Cao, Shuoyang Huang, Yongbin Zheng
Front Immunol. 2021 Jul 16 .
影响因子： 6.76
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
suspended in PBS. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600
Testing Rabbit Bleeds By Elisa
antibody diluted in Blocking Buffer for 2 hr at RT. I do tripling dilutions beginning at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP
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