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- 文献和实验
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- 库存:
99
- 英文名:
ScienCell Trypan Blue (0.4%), 50 ml
- 供应商:
北京云肽生物科技有限公司
北京云肽生物作为ScienCell品牌的经销商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。
ScienCell 0203 台盼蓝TB Trypan Blue (0.4%), 50 ml,产品简介:
产品品牌:ScienCell
产品货号:0203
产品名称:ScienCell 0203 台盼蓝TB Trypan Blue (0.4%), 50 ml
产品规格:50 ml
ScienCell研究实验室提供了整套的细胞培养基添加物和其它细胞培养试剂,这些试剂符合较高品质的工业标准。我们还提供各种细胞转染试剂,针对不同类型的细胞,全面优化,提高转染效率,同时提供细胞培养所需要的细胞外基质成分重组蛋白。您的实验是否需要经美国农业部认证的胰蛋白酶/EDTA、无酶的解离液和更多相关试剂?这些我们均可提供,并经过全面测试,保证您的细胞达到较佳生长状态。
ScienCell 0203 台盼蓝TB Trypan Blue (0.4%), 50 ml,产品说明:
台盼蓝(C34H24N6Na4O14S4,MW 960.81)溶液是一种用于区分活细胞和非活细胞的染色剂。非活性细胞将吸收染料并呈现蓝色,而活性细胞将排除染料。细胞应在计数前置于缓冲盐水中的单细胞悬浮液中。由于台盼蓝对血清蛋白的亲和力高于细胞蛋白,因此将细胞悬浮在含有血清的培养基中会产生暗背景。
Trypan Blue (C34H24N6Na4O14S4, MW 960.81) solution is a stain used to distinguish viable from nonviable cells. Nonviable cells will absorb the dye and appear blue, while viable cells will exclude the dye. Cells should be in a single cell suspension in buffered saline before counting. Since trypan blue has a higher affinity for serum protein than for cellular proteins, suspending the cells in medium containing serum will generate a dark background.
以下是ScienCell品牌部分产品货号,如需其他产品请联系本店客服。
| 品牌 | 货号 | 产品名称 | 规格 |
| ScienCell | 0103 | 胰酶/EDTA消化液 0.25% | 100 ml |
| ScienCell | 0123 | 非酶细胞裂解液 ECDS | 100ml |
| ScienCell | 0143 | 无血清细胞冻存液 CFM-sf | 50ml |
| ScienCell | 0173 | 大豆胰蛋白酶抵制剂 STI | 50ml |
| ScienCell | 0183 | 胰酶/EDTA消化液0.05% | 100 ml |
| ScienCell | 0203 | 台盼蓝 TB | 50 ml |
| ScienCell | 0303 | 磷酸盐缓冲液 DPBS | 500ml |
| ScienCell | 0313 | 平衡盐溶液 HBSS | 500ml |
| ScienCell | 0403 | 多聚赖氨酸 PLL | 1 mg/ml |
| ScienCell | 0413 | 多聚赖氨酸 PLL | 10 mg/ml |
| ScienCell | 0423 | 0.2%明胶溶液(无菌) GSN | 100 ml |
| ScienCell | 0503 | 青霉素/链霉素溶液 P/S | 5 ml |
| ScienCell | 0513 | 青霉素/链霉素溶液 P/S | 100 ml |
| ScienCell | 0523 | 抗真菌溶液 AMS | 50 ml |
| ScienCell | 0533 | 抗霉菌溶液 ABAMS | 50 ml |
| ScienCell | 0543 | 嘌呤霉素 PURO-10 | 10ml |
| ScienCell | 0553 | 嘌呤霉素 PURO-1 | 1 ml |
| ScienCell | 0563 | 支原体抑制剂溶液 MIS | 10 ml |
| ScienCell | 0600 | 细胞培养超纯水 CCGW | 500 ml |
| ScienCell | 0803 | 胰岛素铁硒传递蛋白 ITS | 10 ml |
| ScienCell | 0813 | 谷氨酰胺溶液 L-Glu | 100ml |
| ScienCell | 0823 | 非必需氨基酸100X NEAA | 100 ml |
| ScienCell | 0863 | (0.2%)肝素 HEP | 5 ml |
| ScienCell | 0893 | 葡萄糖溶液 GS | 100 ml |
| ScienCell | 5803 | 人胚胎干细胞解离液 StemDS | 100 ml |
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文献和实验果蝇细胞的培养实验方法(Culturing Drosophila cells)
. COUNTING Remove ~0.5 ml cells from flask (trypsinize cells if necessary) into eppendorf. Dilute cells into Trypan Blue according to estimated density. (confluent cultures try 30:70 µl to 50:50 µl of cells:TB). Transfer 10 - 12 µl cells
cells from flask (trypsinize cells if necessary) into eppendorf. Dilute cells into Trypan Blue according to estimated density. (confluent cultures try 30:70 µl to 50:50 µl of cells:TB). Transfer 10 - 12 µl cells to haemocytometer. Count cells
How do you culture your Drosophila cells
~0.5 ml cells from flask (trypsinize cells if necessary) into eppendorf. Dilute cells into Trypan Blue according to estimated density. (confluent cultures try 30:70 µl to 50:50 µl of cells:TB). Transfer 10 - 12 µl cells to haemocytometer. Count
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