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- 技术资料
- 库存:
99
- 英文名:
APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl
- 供应商:
北京云肽生物科技有限公司
北京云肽生物科技有限公司作为 BioLegend 的特约经销商,为广大客户提供原装正品,货期短,折扣好的服务准则。
美国 BioLegend 公司位于美国加州,致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend 虽然成立的时间不长,但它聚集了一批来自 PharMingen 公司的专业人士,建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势,以及与世界知名实验室和研究所的良好合作关系,以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。
BioLegend 公司以其专业技术力量为保证,提供各种质量卓越的产品,并不断地开发新产品,以满足飞速发展的生命科学研究的需要。
BioLegend 400128 APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl
产品简介:
产品品牌:BioLegend
产品货号:400128
产品名称:BioLegend 400128 APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl
产品规格:100 tests
产品说明:
Clone MOPC-21 (See other available formats)
Regulatory Status RUO
Isotype Mouse IgG1, κ
描述
MOPC-21免疫球蛋白的特异性未知。该抗体的同种型为小鼠IgG1,κ。在对各种静止、活化、活和固定的小鼠、大鼠和人体组织进行筛选后,选择该抗体作为同种型对照。
The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Product Details
Antibody Type Monoclonal
Host Species Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The immunoglobulin was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application FC - Quality tested
ICFC - Verified
Recommended Usage
Each lot of this mouse IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody.
Excitation Laser Red Laser (633 nm)
Application Notes
Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)
Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
以下是 BioLegend 品牌部分产品,如需其他产品请联系本公司销售。
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BioLegend 323031 Brilliant Violet 605™ anti-human CD15 (SSEA-1) W6D3 25 tests
BioLegend 323032 Brilliant Violet 605™ anti-human CD15 (SSEA-1) W6D3 100 tests
BioLegend 323033 Brilliant Violet 650™ anti-human CD15 (SSEA-1) W6D3 25 tests
BioLegend 323034 Brilliant Violet 650™ anti-human CD15 (SSEA-1) W6D3 100 tests
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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
Determination of class and subclass of monoclonal antibodies (MAbs) is important because several immunochemical and functional properties of antibodies depend on the isotype. Knowledge of the light-chain isotype (κ for 95% or λ
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
2 (2a/2b), IgG3, IgG4 IgA:IgA1, IgA2 IgM: IgM1, IgM2 IgD/ IgE : not found 2.型和亚型(根据轻链C区抗原特异性不同分型) 型(type): κ(kappa)型 (human 60%/mouse 95%);λ(lambda)型 (human 40%/mouse 5%) 同一天然Ig分子中两条轻链的型必然相同/同一个体内可有不同型的Ig亚型(λ链)(subtype
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